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yeast strain y187  (TaKaRa)


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    Structured Review

    TaKaRa yeast strain y187
    DNA binding mutant proteins display minimal loss of self-association in the yeast two-hybrid assay budding yeast strain <t>Y187</t> was transformed with plasmids pGBT9 and pGAD424, or derivatives containing wild-type or mutant HsRAD52 sequences. Transformants were grown and whole cell extracts prepared. β-galactosidase specific activities, in Miller units were determined with extracts from a minimum of 10 independent cultures of each transformant. Mean specific activities and 95% confidence intervals are plotted. Fold differences from the pGBT9/pGAD424 negative control appear in boxes above the plots of the mean specific activities.
    Yeast Strain Y187, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1208 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yeast strain y187/product/TaKaRa
    Average 97 stars, based on 1208 article reviews
    yeast strain y187 - by Bioz Stars, 2026-02
    97/100 stars

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    1) Product Images from "Mutations altering the DNA binding domains of the human RAD52 protein exert distinct effects on homologous recombination repair in Saccharomyces cerevisiae"

    Article Title: Mutations altering the DNA binding domains of the human RAD52 protein exert distinct effects on homologous recombination repair in Saccharomyces cerevisiae

    Journal: G3: Genes | Genomes | Genetics

    doi: 10.1093/g3journal/jkaf282

    DNA binding mutant proteins display minimal loss of self-association in the yeast two-hybrid assay budding yeast strain Y187 was transformed with plasmids pGBT9 and pGAD424, or derivatives containing wild-type or mutant HsRAD52 sequences. Transformants were grown and whole cell extracts prepared. β-galactosidase specific activities, in Miller units were determined with extracts from a minimum of 10 independent cultures of each transformant. Mean specific activities and 95% confidence intervals are plotted. Fold differences from the pGBT9/pGAD424 negative control appear in boxes above the plots of the mean specific activities.
    Figure Legend Snippet: DNA binding mutant proteins display minimal loss of self-association in the yeast two-hybrid assay budding yeast strain Y187 was transformed with plasmids pGBT9 and pGAD424, or derivatives containing wild-type or mutant HsRAD52 sequences. Transformants were grown and whole cell extracts prepared. β-galactosidase specific activities, in Miller units were determined with extracts from a minimum of 10 independent cultures of each transformant. Mean specific activities and 95% confidence intervals are plotted. Fold differences from the pGBT9/pGAD424 negative control appear in boxes above the plots of the mean specific activities.

    Techniques Used: Binding Assay, Mutagenesis, Y2H Assay, Transformation Assay, Negative Control



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    yeast strain y187  (TaKaRa)
    97
    TaKaRa yeast strain y187
    DNA binding mutant proteins display minimal loss of self-association in the yeast two-hybrid assay budding yeast strain <t>Y187</t> was transformed with plasmids pGBT9 and pGAD424, or derivatives containing wild-type or mutant HsRAD52 sequences. Transformants were grown and whole cell extracts prepared. β-galactosidase specific activities, in Miller units were determined with extracts from a minimum of 10 independent cultures of each transformant. Mean specific activities and 95% confidence intervals are plotted. Fold differences from the pGBT9/pGAD424 negative control appear in boxes above the plots of the mean specific activities.
    Yeast Strain Y187, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/yeast strain y187/product/TaKaRa
    Average 97 stars, based on 1 article reviews
    yeast strain y187 - by Bioz Stars, 2026-02
    97/100 stars
    		  Buy from Supplier
    y187 yeast strain  (TaKaRa)
    95
    TaKaRa y187 yeast strain
    DNA binding mutant proteins display minimal loss of self-association in the yeast two-hybrid assay budding yeast strain <t>Y187</t> was transformed with plasmids pGBT9 and pGAD424, or derivatives containing wild-type or mutant HsRAD52 sequences. Transformants were grown and whole cell extracts prepared. β-galactosidase specific activities, in Miller units were determined with extracts from a minimum of 10 independent cultures of each transformant. Mean specific activities and 95% confidence intervals are plotted. Fold differences from the pGBT9/pGAD424 negative control appear in boxes above the plots of the mean specific activities.
    Y187 Yeast Strain, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/y187 yeast strain/product/TaKaRa
    Average 95 stars, based on 1 article reviews
    y187 yeast strain - by Bioz Stars, 2026-02
    95/100 stars
    		  Buy from Supplier

    Image Search Results


    DNA binding mutant proteins display minimal loss of self-association in the yeast two-hybrid assay budding yeast strain Y187 was transformed with plasmids pGBT9 and pGAD424, or derivatives containing wild-type or mutant HsRAD52 sequences. Transformants were grown and whole cell extracts prepared. β-galactosidase specific activities, in Miller units were determined with extracts from a minimum of 10 independent cultures of each transformant. Mean specific activities and 95% confidence intervals are plotted. Fold differences from the pGBT9/pGAD424 negative control appear in boxes above the plots of the mean specific activities.

    Journal: G3: Genes | Genomes | Genetics

    Article Title: Mutations altering the DNA binding domains of the human RAD52 protein exert distinct effects on homologous recombination repair in Saccharomyces cerevisiae

    doi: 10.1093/g3journal/jkaf282

    Figure Lengend Snippet: DNA binding mutant proteins display minimal loss of self-association in the yeast two-hybrid assay budding yeast strain Y187 was transformed with plasmids pGBT9 and pGAD424, or derivatives containing wild-type or mutant HsRAD52 sequences. Transformants were grown and whole cell extracts prepared. β-galactosidase specific activities, in Miller units were determined with extracts from a minimum of 10 independent cultures of each transformant. Mean specific activities and 95% confidence intervals are plotted. Fold differences from the pGBT9/pGAD424 negative control appear in boxes above the plots of the mean specific activities.

    Article Snippet: Strains used in yeast two-hybrid analysis were constructed by transforming the appropriate plasmids into the yeast strain Y187 (Clontech, Mountain View, CA, USA).

    Techniques: Binding Assay, Mutagenesis, Y2H Assay, Transformation Assay, Negative Control